{"id":106002,"date":"2024-12-05T05:26:51","date_gmt":"2024-12-05T11:26:51","guid":{"rendered":"https:\/\/milesfortis.com\/?p=106002"},"modified":"2024-12-05T05:33:55","modified_gmt":"2024-12-05T11:33:55","slug":"106002","status":"publish","type":"post","link":"https:\/\/milesfortis.com\/?p=106002","title":{"rendered":""},"content":{"rendered":"

BLUF
\nPEER REVIEWED SCIENCE SAYS THE BIOTECH VACCINES HAVE LOTS OF DNA IN THEM …..IT CAN REPLICATE AND IT COULD BE TRANSMITTED FROM ONE PERSON TO ANOTHER<\/em><\/strong><\/p>\n

The most comprehensive study on Vax DNA sails through peer review.<\/a><\/p>\n

Ulrike Kammerer, Verena Schulz and Klaus Steger just published a bombshell in a\u00a0peer reviewed journal.<\/a><\/p>\n

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I was invited to peer review this paper but declined as I felt having me review this would be viewed as inside baseball. For this to stand the test of time, we cannot resort to the boys club bullshit exhibited by the Faucists.<\/p>\n

There are several aspects of this paper that are worth reviewing in detail. The paper not only settles some of the methods debate ongoing in the field regarding how to best quantitate the DNA in these vaccines, but it also transfects HEK cells with the vaccines and demonstrates the spike expresses for longer than 7 days and it doesn\u2019t stay parked on the cell membrane. It get packaged and into exosomes and presumably exported all over the body.<\/strong><\/p>\n

This is an important finding as exosomes are exhaled and exported to the surface of the skin. This has major implications for the shedding story. If these exosomes contain plasmids, then the gig is up. That implies transmissible and potentially replication competent DNA that encodes spike protein and SV40 components<\/strong>. Its not clear if these plasmids will express spike protein in mammalian cells as the T7 promoter should only be active in bacterial cells but your body is loaded with bacteria and bactofection is a thing.<\/p>\n

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Other plasmids have also been discovered in people encoding nucleocapsid protein.\u00a0<\/strong>Have a look at the\u00a0Beck et al paper\u00a0<\/a>which documents a lab leak in Seattle that hit lab staff and their housemates with\u00a0pcDNA3-SARs-CoV-2-N\u00a0<\/a>expressing plasmids. These plasmids look a lot like Pfizers plasmids, complete with SV40 components but also have a CMV promoter that ensures the nucleocapsid plasmid is expressed in mammalian cells (different than T7 found in BNT162b2). These plasmids are in use all over the world and some encode\u00a0spike protein\u00a0<\/a>and likely also leak as they did in Seattle. No one is paying attention to this leak risk despite the Beck paper in Seattle.<\/p>\n

Ulrike\u2019s paper touches on this. These are\u00a0shuttle vectors<\/strong>\u00a0which is nice way of saying they are\u00a0Zoonotic plasmids<\/strong>\u00a0that can hop between bacteria and mammalian cells lines and replicate in both.<\/p>\n

Thats an evolutionary leap most people don\u2019t appreciate. After all, SV40 was a simian virus and normally doesn\u2019t infect bacteria but once you cut and paste the most functional pieces of it in pUC like plasmids you can get this DNA to now replicate in bacteria and mammalian cells extending its evolutionary reach and modes of transmission.<\/p>\n

So lets get to the meat of the paper..<\/p>\n

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They quantitate intracellular spike protein which is highest by day 5 but still higher than day 1 out at 7 days post transfection. This is\u00a0Figure C.<\/strong>\u00a0Then in\u00a0Figure D\u00a0<\/strong>they show the spike protein exported from the cell keeps rising by day 7. This wasn\u2019t supposed to happen. The powerpoint model of this system was that the spike would be anchored to cell membrane and stay localized to the muscle cells which don\u2019t divide and immunity would be built up in the muscles and be gone in 48 hours.<\/p>\n

Instead we have HEK cells turning into spike export factories.<\/p>\n

They then demonstrate these spike proteins are actually in exosomes.<\/p>\n

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This should raise major alarm bells as the mechanism of biodistribution is now more complicated than simple LNP dilution. I would love to PCR some of these exosomes.<\/p>\n

They then perform the most rational and comprehensive quantitation study I\u2019ve seen to date.<\/p>\n

They take 3 different fluorometry kits (PicoGreen, Qubit, and AccuBlue) and measure the DNA concentration before and after the use of RNaseA. RNaseA eliminates the RNA so the cross talk criticisms are neutralized.<\/p>\n

Recall the TGA has been disregarding the Konig work as it didn\u2019t use RNaseA. Speicher et al did use RNaseA in his Australian vial study but they tried to ignore that because it wasn\u2019t peer reviewed. I actually reviewed it privately. Now that debate is settled and in the peer reviewed literature. It lines up with work that has been submitted for publication by others. Consensus is building.<\/p>\n

Note the Fluorometry for the RNA comes in right as expected (Figure A). Keep that in mind. Thats an important detail for dismantling the Kaiser et al study.\u00a0Figure B and C<\/strong>\u00a0shows AccuBlu has the least cross talk with RNA (This is what we have been using) and the Qubit has the most cross talk signal (signal without RNaseA – Signal with RNaseA). After RNaseA digestion the signals for all assays fall to only be measuring the DNA.<\/p>\n

This is what we have been seeing as well. The signal drops an order of magnitude after RNaseA but still remains over the limit.<\/p>\n

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They also go on to demonstrate how important it is to use Triton-X-100 to open up the LNPs or you will under measure the DNA. We get an additional boost if we use Triton-X-100 and 95C heat but this might decay the RNA.<\/p>\n

I was also attacked online for using AccuBlu as this is often thought to be a knock-off Qubit kit. What these Twitter clowns didn\u2019t respect is that I was a Scientific Fellow at Life Tech which developed the Qubit and I had inside knowledge of how all of these kits performed next to one another as we heavily relied on them to feed the SOLiD sequencers. SOLiD sequencers have to have the most precise DNA and RNA measurements as you must dilute your samples down to single molecule digital PCR levels and if you screw this up you toast a $1000 emulsion PCR reaction and potentially a $10,000 Sequencing run. And often the DNA libraries are very small molecules and can contain primer dimers, so understanding how these quantification dyes performed with small DNA and RNAs was critical to success of our customers. This next generation sequencer capture 30% of the market when Lifetech acquired ABI in 2008. So we had to nail DNA and RNA quant and we knew the parameters at which these tools fail and were not disclosed in their marketing material.<\/p>\n

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But this dismantling won\u2019t stop the pipette-free peanut gallery from moving the goal posts yet again.<\/p>\n

\u201cSo what if its over the limit. Its so small the cells will destroy it!\u201d<\/p><\/blockquote>\n

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Yes, the clowns were wrong about that as well. The DNA is present after washing the cells. So it is inside the cells and even 362bp amplicons can be generated from Spike\u00a0Figure 3B<\/strong>.<\/p>\n

The DNA is in the cells and 362bp amplicons can be amplified suggesting its not getting destroyed by the cells but likely triggering cGAS-STING.<\/p>\n

Kwon et al\u00a0<\/a>shows how that can lead to Cancer.<\/p>\n

Of course this shouldn\u2019t surprise any of us given the US Department of HHS spent $1B buying off the media and paid influencers to reduce vaccine hesitancy. Shame! I bet this party ends with RFKJr.<\/p>\n

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